Identification of candida albicans in oral candidiasis by germ tube test

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Original Article

Author Details : A Jain, M Barua, Kiran Kumar, Devi Charan Shetty*

Volume : 1, Issue : 1, Year : 2013

Article Page : 29-33

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Abstract

Background: Oral candidiasis is caused by overgrowth or infection of oral cavity by yeast like fungus, Candida. Out of pathogenic 20 species, C. albicans is most common species associated with oral candidiasis. Oral candidiasis marks systemic disease such as diabetes mellitus and commonly encountered among the immunocompromised patients with HIV as opportunistic infections. It appears as white or cream colour patches and often bleeds if rubbed or scraped. Though the diagnosis of oral candidiasis can be made clinically, laboratory procedures and mycological cultures have to be used for the confirmation of the diagnosis.

Aims: To assess the association of C. albicans species with Oral candidiasis using Germ Tube Test.

Method and Result Analysis: The study was conducted in Department of Oral Pathology and Microbiology and Department of Oral Medicine and Radiology ITS-CDSR. A total of 25 patients clinically suggestive of oral candidiasis were selected. After obtaining consent, oral scrapings were collected and subjected to see the presence of fungal element using 10% KOH. Further the samples were inoculated on Sabouraud Dextrose Agar and Blood Agar for fungal isolation and identification of Candida albicans using Germ Tube Test.

Results: The number of cases associated with C. albicans were positive for germ tubetestand analysed statistically

Keywords: Candidiasis, Germ Tube Test, Sabouraud Dextrose Agar, 10% KOH.

How to cite : Jain A, Barua M, Kumar K, Shetty D C, Identification of candida albicans in oral candidiasis by germ tube test. J Dent Spec 2013;1(1):29-33

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