Synergistic antibacterial action of nanoparticle-Enhanced royal jelly and pulp capping agents against Streptococcus mutans

• Author Details:   
• Ketaki Turbatmath ^*
• Sonali Sharma
• Lakshmipathy Muthukrishnan

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Introduction: Nanoparticles can have natural antimicrobial capabilities or be engineered to transport antibacterial chemicals, hence increasing their efficacy. By integrating nanoparticles into these goods, we can leverage the synergistic effects of nanoparticles and bioactive chemicals found in apiarian products, resulting in increased antibacterial activity.
Materials and Methods: Royal jelly was obtained from Queenbees Pvt Ltd, located in Chennai, Tamil Nadu, India. Calcium Hydroxide in the form of Dycal (Dentsply) was used as the base material. The experimental groups were categorized as follows: Group A—Dycal with royal jelly extract, Group B—Dycal with nanoparticle-incorporated royal jelly, and Group C—Dycal with nanoparticle-incorporated calcium hydroxide.
Nanoparticles were synthesized and examined for FTIR, SEM, EDAX, and antibiofilm testing.
Results: Group 1 includes alkanes (702.5), aromatic groups (615.50), and carbonyl groups (ketones and amides) (1636.97). Group 2: The silver nanoparticles included in royal jelly have function and hydroxyl groups. Group 3: The presence of hydroxyl (3300.05) and carbonyl groups (1700). SEM investigation reveals the crystalline structure of nanoparticles spread uniformly in royal jelly. The EDAX analysis reveals that the Royal jelly sample has a considerable amount of silver integrated into the matrix.
Conclusion: Silver Nanoparticle incorporated royal jelly demonstrated maximum biofilm suppression against Streptococcus mutans. The results from FTIR, SEM, and EDAX confirm the successful incorporation of silver nanoparticles into royal jelly, demonstrating their potential for enhanced antibacterial efficacy.

Keywords: Apiarian product, Indigenously developed, Innovative pulp capping agent, Nanoparticle synthesis, Pulp capping agent.